Two further alkaline phosphatase staining methods for immunohistochemistry.
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چکیده
منابع مشابه
Quantification and comparison of bone-specific alkaline phosphatase with two methods in normal and paget’s specimens
Background: Bone-specific alkaline phosphatase (BAP) is synthesized by the osteoblasts and is presumed to be involved in the calcification of bone matrix, though its precise role in the formation process is unknown. The aim of the present study was to measure the BAP activity in Paget's and normal specimens by two different techniques. Methods: Total ALP (TAP) as well as BAP activity-measuring ...
متن کاملPlasma alkaline phosphatase assay: interconversion of results by two methods.
Two methods for measuring plasma alkaline phosphatase activity are compared: one makes use of phenyl phosphate, carbonate-bicarbonate buffer, and continuous-flow methodology; the other of p-nitrophenyl phosphate, diethanolamine buffer, and reaction-rate analysis. Results by the methods correlate well (r = 0.98) over a wide range of values (up to 10-fold the upper limit of normal). A factor can ...
متن کاملImproved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence.
AIMS To develop and immunohistochemical staining method for cryostat and paraffin wax sections so that two different antigens in the same section of tissues could be detected by combining immunoenzyme and immunofluorescence techniques. METHODS This double immunohistochemical staining method combines alkaline phosphatase-anti-alkaline phosphatase (APAAP) using New Fuchsin as a chromogen and in...
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OYA, M., SHIBATA, R., KIDO, A. and KOMATSU, N. A Further Study on the Polymorphism of Placental Alkaline Phosphatase in Japanese. Tohoku J. exp. Med., 1985, 146 (2), 175-179 Polymorphism of placental alkaline phosphatase (PL) was investigated in 120 Japanese placentae by means of starch gel electrophoresis and an improved staining method for the isoenzymes using naphthol AS-MX phosphate as subs...
متن کاملDouble staining immunohistochemistry
This article summarized immunohistochemistry methods generally used in research laboratories and clinic including direct immune staining, indirect immune staining, enzyme method, fluorescence method, APC method and PAP method.
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1984
ISSN: 0021-9746
DOI: 10.1136/jcp.37.9.1078